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 CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae
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CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae

автор jinghua q. издательство Книга по Требованию Другие общетехнические дисциплины
Cтраниц 164        мягкая обложка       
Аннотация:Homocitrate synthase (HCS1) (acetyl-coenzyme A: 2-ketoglutarate C-transferase; E. C. 2. 3. 3. 14) catalyzes the first and regulated step in the a-aminoadipate pathway for lysine synthesis. The pH dependence of the kinetic parameters, isotope effects, and dissociation constants for competitive inhibitors are used to probe the chemical mechanism of HCS. Data combined with a constant pH molecular dynamics simulation study suggest a catalytic dyad, comprised of Glu-155 and His-309, functions as a general base to deprotonate the methyl group of AcCoA. A general acid-base chemical mechanism is proposed. site-directed mutagenesis was used to change the three active site residues of HCS, and the resulting mutant enzymes were characterized using initial velocity studies, the pH dependence of the kinetic parameters and isotope effects.
Автор: jinghua q.
Издательство: Книга по Требованию
Год:2009
Цена:
2746 руб
Нет в наличии
Поиск: CHEMICAL MECHANISM OF HOMOCITRATE SYNTHASE FROM Saccharomyces cerevisiae
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
Ортомолекулярная медицина Orthomol Устрание причин заболеваний. Ортомоль это новый метод для лечения и профилактики болезней. Orthomol - ортомолекулярная медицина.
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Наиболие часто в качестве клетки-хозяина используют кишечную палочку, однако применяют и другие бактерии, а так же дрожжи (Saccharomyces cerevisiae),животные и растительные клетки. Система вектор-хозяин   Не может быть произвольной: вектор подгоняется к клетке-хозяину,его выбор зависет от видовой специфичности и целей иследователя. Существует три пути селекции рекДНК : генетический (по маркерам, с помощью избирательных сред), иммунохимическй и гибридизационный с мечеными ДНК и РНК. РекДНК характеризуют физическим картированием (расщепление рекстриктазами и электрофорез фрагментов в геле) и анализом первичной структуры. В результате интенсивного развития методов генной инженерии получены клоны многих генов рибосомальной, транспортной и 5S PHK, гистонов, глобина мыши,кролика, человека, коллагена, овальбумина, инсулина человека, а совсем недавнее открытие- расшировка генома человка, сделанное в январе двухтысячного года, позволет в скором будущем клонировать человека.На основе генной инженерии возникла отрасль фармацевтической промышленности, называемая “индустрией ДНК” и представляющая собой одну из современых ветвей биотехнологии.
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