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 Metabolic Engineering of Bacillus. for Enhanced Product and Cellular Yield
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Metabolic Engineering of Bacillus. for Enhanced Product and Cellular Yield

автор Zhiwei P. издательство Книга по Требованию Экономическая теория
Cтраниц 144        мягкая обложка       
Аннотация:Metabolic engineering provides a powerful approach to optimize the cellular activities and improve product yields by genetically manipulating specific metabolic pathways. Recombinant protein was improved two-fold by the PYK mutation due to low acetate formation and longer production time in the pyk mutant. A combination of PYK mutation and additional mutations in the folic acid synthesis pathway resulted in an 8-fold increase in folic acid production. In this study, an inducible PYK (iPYK) mutant of B. subtilis was constructed based on the outcome of metabolic modeling. The results demonstrate that good cell growth rate and low acetate formation can be attained at an appropriate PYK expression level. In Addition, the PYK mutation was explored as an efficient way to improve the production of recombinant protein and valuable metabolites such as folic acid. Microbial cultures usually produce a significant amount of acidic byproducts which can repress cell growth and product synthesis.
Автор: Zhiwei P.
Издательство: Книга по Требованию
Год:2009
Цена:
2384 руб
Нет в наличии
Поиск: Metabolic Engineering of Bacillus. for Enhanced Product and Cellular Yield
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
Ортомолекулярная медицина Orthomol Устрание причин заболеваний. Ортомоль это новый метод для лечения и профилактики болезней. Orthomol - ортомолекулярная медицина.
Добыча золота методами геотехнологии

Бактериальное выщелачивание позволяет решить проблему переработки труднообогатимых руд. Проводится поиск новых видов микроорганизмов, которые способны функционировать не только в кислой, но и в нейтральной и в щелочной средах. Как показали опыты, проведенные в СССР и в Индии, специальное внесение бактерий в рудную массу необязательно. Путем адаптации с использованием различных мутагенных факторов можно получить культуру со свойствами, необходимыми для ее промышленного применения. Пионерами исследований по бактериальному выщелачиванию золота были институт Пастера (Франция) и университет г. Дакар (Сенегал). Информация об этих работах появилась в печати в 60-х годах XX века. Бактериальные методы извлечения золота из руд базируются на результатах изучения микрофлоры крупных золоторудных месторождений, позволивших выделить культуры доминирующих видов бактерий и грибов. Установлено, что повышенной активностью в процессе растворения золота обладают представители родов Bacillus, Bac erium, Chromobac erium, а также полученные на основе индуцированного мутагенеза штаммы бактерий Bac. mese ericus iger 12 и 129.
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