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 Die Initiation der DNA-Replikation. Biochemische Charakterisierung der humanen DNA Replikation in vitro
Наименование:

Die Initiation der DNA-Replikation. Biochemische Charakterisierung der humanen DNA Replikation in vitro

автор Jens B. издательство Книга по Требованию Органическая химия
Cтраниц 120        мягкая обложка       
Аннотация:Die Aufklaerung der dabei zugrunde liegenden Mechanismen wurde durch die Entwicklung von in vitro Replikationssystemen entscheidend vorangetrieben. Mit Hilfe dieses Ansatzes werden regulatorische Mechanismen, die fuer die zellzyklusabhaengige Initiation der DNA- Replikation essentiell sind charakterisiert. Bis heute konnte jedoch kein vollstaendig loesliches humanes System etabliert werden, dass es erlaubt, die Ausbildung des prae-Replikationskomplexes (pre-RC) und die Initiation der DNA-Replikation zu untersuchen. In dieser Arbeit wird ein solches in vitro Replikationssystem vorgestellt, dass auf Extrakten aus HeLa- Zellen basiert. Zudem werden die einzelnen Schritte bei der sequentiellen Ausbildung des pre-RCs unabhaengig von der Initiation der DNA-Replikation in Plasmid- Bindestudien biochemisch untersucht. Dieses Ereignis der DNA-Replikation unterliegt in eukaryotischen Zellen einer strengen Regulation. Bevor sich eine Zelle teilt muss das Erbmaterial vollstaendig dupliziert werden. Mit Hilfe rekombinanter Proteine wird die Rolle von Orc6 bei der Bindung des humanen „Origin recognition Complex“ (ORC) an DNA und der pre-RC Ausbildung naeher charakterisiert.
Автор: Jens B.
Издательство: Книга по Требованию
Год:2009
Цена:
2361 руб
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Поиск: Die Initiation der DNA-Replikation. Biochemische Charakterisierung der humanen DNA Replikation in vitro
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