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 Studied by Electrospray Ionization Tandem Mass Spectrometry Gas-Phase Dissociation of Oligonucleotides.
Наименование:

Studied by Electrospray Ionization Tandem Mass Spectrometry Gas-Phase Dissociation of Oligonucleotides.

автор Jan T. издательство Книга по Требованию Физическая химия
Cтраниц 88        мягкая обложка       
Аннотация:Their high specificity offers promise for the development of new drugs for protein induced diseases. Experiments with 2' -modified oligonucleotides were performed to investigate the reasons for the different gas-phase fragmentation behavior of DNA and RNA. Oligonucleotides with C-glycosidic bound biphenyl substituents were analyzed to study the influence of the nucleobase in gas-phase dissociation. A mechanism for RNA gas-phase dissociation, in accordance with all experimental data, is presented. The various unnatural oligonucleotides are not compatible with the classical sequencing techniques. In this work unmodified DNA, RNA and several ribose or base modified oligonucleotides were analyzed to elucidate the gas-phase dissociation mechanism. Mass spectrometry has the potential to achieve these requirements. Oligonucleotides have great potential in diagnostic and therapeutic applications. However, mechanistic aspects of oligonucleotide dissociation have to be clarified first. Therefore, new methods for fast and unambiguous sequence verification are needed. Several modifications were introduced in oligonucleotides to improve nuclease resistance, binding affinity and specificity.
Автор: Jan T.
Издательство: Книга по Требованию
Год:2009
Цена:
2361 руб
Нет в наличии
Поиск: Studied by Electrospray Ionization Tandem Mass Spectrometry Gas-Phase Dissociation of Oligonucleotides.
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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