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 OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER. A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
Наименование:

OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER. A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS

автор Cheng P. издательство Книга по Требованию Механика. Динамика
Cтраниц 88        мягкая обложка       
Аннотация:Pore size was found dependent on incident laser intensity. This dissertation presents a novel technique of delivering membrane impermeable molecules into single living cells with the assistance of femtosecond (fs) near-infrared (NIR) laser pulses. The connections between laser parameters and cell responses were explored. The conclusion was made that laser-induced breakdown and plasma-induced ablation in cell membrane are the physical principles that govern the process of fs NIR opto-injection. Dye uptake rate of the target cells was observed to depend on incident laser intensity. In this work, the system for fs NIR opto-injection was designed and built. Successful fs NIR opto-injection has been performed on several cell systems including single mammalian cells, marine animal eggs, and human cancer cells cultured in a tissue-like environment. This technique promises several major advantages, namely, very high transfection efficiency, high cell survival rate (? 100%) and fully preserved cell viabilities.
Автор: Cheng P.
Издательство: Книга по Требованию
Год:2008
Цена:
2007 руб
Нет в наличии
Поиск: OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER. A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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