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 A Green Fluorescent Protein-Based Purification System for Plant Cells. Optimizing Yield and Recovery of Recombinant Proteins from Plants
Наименование:

A Green Fluorescent Protein-Based Purification System for Plant Cells. Optimizing Yield and Recovery of Recombinant Proteins from Plants

автор Gabriel P. издательство Книга по Требованию Книги
Cтраниц 148        мягкая обложка       
Аннотация:This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein. Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps.
Автор: Gabriel P.
Издательство: Книга по Требованию
Год:2009
Цена:
2415 руб
Нет в наличии
Поиск: A Green Fluorescent Protein-Based Purification System for Plant Cells. Optimizing Yield and Recovery of Recombinant Proteins from Plants
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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