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 Synthetic Green Fluorescent Protein Chromophores. PHOTOCHEMICAL AND PHOTOPHYSICAL STUDIES OF SYNTHETIC DERIVATIVES OF THE GREEN FLUORESCENT PROTEIN CHROMOPHORE
Наименование:

Synthetic Green Fluorescent Protein Chromophores. PHOTOCHEMICAL AND PHOTOPHYSICAL STUDIES OF SYNTHETIC DERIVATIVES OF THE GREEN FLUORESCENT PROTEIN CHROMOPHORE

автор Jian D. издательство Книга по Требованию География
Cтраниц 128        мягкая обложка       
Аннотация:Finally, a hydroxyl substituent at the meta position shows enhanced charge transfer and greater acidity in the excited state. Cis/trans isomerization is thought to be responsible for blinking behavior in fluorescent protein; however, the mechanism of the thermal reverse isomerization is more problematic. We found some O-alkyl GFP chromophore derivatives exhibit large fluorescent enhancement in the solid state. Using Hammett plot studies, we conceived, for the first time, a novel nucleophilic addition/elimination mechanism. As a result, we have demonstrated that the fast quenching of the excited state by internal conversion to the ground state is much slower in meta- than in para-HOBDI derivatives. This allows studies of this ultrafast intermolecular ESPT that competes with isomerization. We first compared their optical properties between solution and solid state. GFP is widely used as a biological marker because of its superb fluorescence properties. We have synthesized GFP chromophore and its derivatives, and their photochemistry and photophysics were investigated using various steady-state and time-resolved techniques.
Автор: Jian D.
Издательство: Книга по Требованию
Год:2009
Цена:
2415 руб
Нет в наличии
Поиск: Synthetic Green Fluorescent Protein Chromophores. PHOTOCHEMICAL AND PHOTOPHYSICAL STUDIES OF SYNTHETIC DERIVATIVES OF THE GREEN FLUORESCENT PROTEIN CHROMOPHORE


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