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 A Novel Bioassay Chamber for Autologous Endothelial Cell Growth. Optimized for Cell Viability and Density on Various Engineered Substrates
Наименование:

A Novel Bioassay Chamber for Autologous Endothelial Cell Growth. Optimized for Cell Viability and Density on Various Engineered Substrates

автор David R. издательство Книга по Требованию Другие общетехнические дисциплины
Cтраниц 176        мягкая обложка       
Аннотация:This leads to replacement failure. One major difficulty involves the lack of vasculature within these scaffolds. Long term culture increased cell viability without affecting density. We take the approach to fabricate a scaffold that directs endothelial cell (EC) migration to form planar capillary networks. Tissue engineering is an interdisciplinary field which focuses on scaffold design to aid in tissue replacement. Results indicated that ECs prefer to grow along fibers with a 1-5 micron diameter. Murine aortas were dissected, cannulated and perfused at low flow rates. This work should help elucidate a new method to engineer blood vessels and should be useful for anyone interested in optimizing biomaterial scaffolds for use with ECs. Here, scaffold composition, surface chemistry and shear stress were used to direct migration. A novel bioassay chamber was designed to optimize EC growth from an autologous donor source. In vivo, a combination of topological, mechanical and chemical cues dictates migration. Experiments were designed to optimize electrospun scaffolds for ECs. Cell density was unrelated to flow rate, but viability was enhanced with higher flow.
Автор: David R.
Издательство: Книга по Требованию
Год:2009
Цена:
2783 руб
Нет в наличии
Поиск: A Novel Bioassay Chamber for Autologous Endothelial Cell Growth. Optimized for Cell Viability and Density on Various Engineered Substrates
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
Ортомолекулярная медицина Orthomol Устрание причин заболеваний. Ортомоль это новый метод для лечения и профилактики болезней. Orthomol - ортомолекулярная медицина.
Целлофан

Удельный расход основных видов сырья при производстве целлофана в таблице 1. Таблица 1. Удельный расход основных видов сырья на производстве целлофана в кг на 1 кг продукции Компонент Расход Целлюлоза 90%-ная, абсолютно сухая 0,90-0,91 Едкий натр (в пересчете на 100%-ный) 0,65-0,8 Сероуглерод, ректификат без рекуперации 0,28 с рекуперацией 0,17 Серная кислота (в пересчете на 100%-ную) при частичной регенерации 1,3-1,4 при полной регенерации 1,25-1,27 Получение вискозы Получение вискозы является первой стадией технологического процесса получения вискозной пленки и волокон. Эта стадия производства включает следующие основные процессы: . получение щелочной целлюлозы; . приготовление рабочих растворов щелочи; . получение ксантогената целлюлозы и его растворение; . подготовку вискозы к формованию. Очищенная природная целлюлоза обрабатывается избытком концентрированного гидроксида натрия: Cell(OH aOH ( Cell(O a H2O Cell aOH ( Cell( aOH После удаления избытка ее комки растирают и полученную массу выдерживают в тщательно контролируемых условиях.
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