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 Extent of HIV-1 drug resistance among drug naive populations of Kenya. HIV drug resistance among drug naive populations
Наименование:

Extent of HIV-1 drug resistance among drug naive populations of Kenya. HIV drug resistance among drug naive populations

автор Anthony N. издательство Книга по Требованию Ветеринарный справочник традиционных и нетрадиционных методов лечения
Cтраниц 152        мягкая обложка       
Аннотация:Generated sequences were then compared with reference HIVdrug resistance database (http: // hivdb. stanford. edu/). The successfully amplified products were directly sequenced using the Big Dye® sequence terminator technology (Applied Biosystems®). Background: Development of resistance to antiretroviral medication can occur in persons on antiretroviral therapy, acquisition of already resistant strain in persons who have never taken medication or by natural polymorphism of the virus in vivo. This leads to treatment failure hence complicating management of HIV patients. Objectives: To determined the extent of HIV-1 drug resistance mutations in HIV-1 positive drug naive persons in Kenyan population. Methods: HIV-1 positive plasma samples from seventy-eight (78) drug naive subjects were collected from Health Centers in five provinces in Kenya. HIV-1 viral RNA was extracted using Qiagen® RNA isolation kit and a 697bp size portion of HIV-1 reverse transcriptase (RT) gene amplified by nested PCR using specific primers and amplified products analyzed by gel electrophoresis and visualized under UV light.
Автор: Anthony N.
Издательство: Книга по Требованию
Год:2010
Цена:
2415 руб
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Поиск: Extent of HIV-1 drug resistance among drug naive populations of Kenya. HIV drug resistance among drug naive populations



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