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 The case for monomeric Enzyme with intersubsite cooperativity. The catalytic mechanism of carboxypeptidase A and active site intersubsite cooperativity
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The case for monomeric Enzyme with intersubsite cooperativity. The catalytic mechanism of carboxypeptidase A and active site intersubsite cooperativity

автор abdulkader r. издательство Книга по Требованию Другие общетехнические дисциплины
Cтраниц 96        мягкая обложка       
Аннотация:Multi- inhibitor analysis maps the likely location of substrates within this multi-subsite enzyme in the active catalytic state. Various synthetic (novel and past) substrates were used and studied by applying advanced kinetics. Later studies have substantiated this important aspect in other enzymes (FE: RNase A, 1999; phospholipase, 2001). It is currently believed that this is a common property of several enzymes and proteins that act on biopolymers and membrane lipids. This represents the thesis work dedicated to unraveling the underlying catalytic mechanism of the exopeptidase carboxypeptidase A. Earlier postulated mechanisms (by Cleland et. al. , Lipscomb et. al. , Suh et. al. . . ), based on crystallographic and kinetic data, are experimentally and theoretically critically explored. Further, the study demonstrates a very early case of multi-subsite cooperative interaction in a monomeric enzyme. The synthesis of a novel substrate with unprecedented kinetic features (extreme kcat/Km value) assured excellent compatibility with the transition state structure of the enzyme; permitting the exclusion of several earlier proposed mechanisms.
Автор: abdulkader r.
Издательство: Книга по Требованию
Год:2010
Цена:
2007 руб
Нет в наличии
Поиск: The case for monomeric Enzyme with intersubsite cooperativity. The catalytic mechanism of carboxypeptidase A and active site intersubsite cooperativity
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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