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 A Promising Approach for Molecular Structural Biology and Nanotechnology Structural Studies of SARS Virus Nsp15 and Human Innate Immunity Receptor TLR3. 2D Crystallization and 3D Reconstruction of Biological Macromolecules:
Наименование:

A Promising Approach for Molecular Structural Biology and Nanotechnology Structural Studies of SARS Virus Nsp15 and Human Innate Immunity Receptor TLR3. 2D Crystallization and 3D Reconstruction of Biological Macromolecules:

автор Jingchuan S. издательство Книга по Требованию Общая биология
Cтраниц 136        мягкая обложка       
Аннотация:Single particle analysis can determine protein structures in solution without the need for crystals. 3D structures of several protein complexes had been solved by the single particle method, including IniA from Mycobacterium tuberculosis, Nsp15 and TLR3 ECD. This approach may also have application in nanofabrication, taking advantage of the natural building bloc of proteins and virus. Determining the structures of these proteins is an important step toward understanding pathogenic microbes and our immune system. In this study, several proteins have been crystallized using this technique, including the SARS virus Nsp15 endonuclease and the human Toll-like receptor 3 extracellular domain. Using lipid monolayer technique with Ni-NTA modified lipid, which has high affinity to 6His-tagged proteins, 2D crystal of the protein can be formed at the lipid surface. Combining the high resolution imaging of TEM and efficient computer processing, protein structures in solution or in 2D crystals can be determined. 3D structural determination of biological macromolecules is not only critical to understanding their mechanisms, but also essential in structural based drug discovery.
Автор: Jingchuan S.
Издательство: Книга по Требованию
Год:2008
Цена:
2415 руб
Нет в наличии
Поиск: A Promising Approach for Molecular Structural Biology and Nanotechnology Structural Studies of SARS Virus Nsp15 and Human Innate Immunity Receptor TLR3. 2D Crystallization and 3D Reconstruction of Biological Macromolecules:
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
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