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 An Immunotoxin for the Depletion of Autoreactive B Cells in MS. Design, expression, and characterization of an autoimmunotoxin for the specific depletion of autoreactive B cells in multiple sclerosis
Наименование:

An Immunotoxin for the Depletion of Autoreactive B Cells in MS. Design, expression, and characterization of an autoimmunotoxin for the specific depletion of autoreactive B cells in multiple sclerosis

автор Sigrun K. издательство Книга по Требованию Органическая химия
Cтраниц 80        мягкая обложка       
Аннотация:This hybridoma cell line that served as a very feasible in vitro B cell model is the first step in the determination of the functionality of an immunotoxin. This is the first time that a functional PLP fusion protein was expressed in 293T cells and purified from cell supernatant. The characterisation of the immunotoxin PLP-ETA comprised the verification of the functionality of the expressed protein in the context of the binding specifity and toxicity on a PLP- specific hybridoma cell line. Stable producing 293T cell populations were established for the reference protein PLP that showed a good expression yield and a high purity after purification was obtained. Documenting a high binding specifity and specific toxicity of this novel PLP based immunotoxin on a PLP- reactive hybridoma cell line, this work provides a contribution to the B cell specific therapies with immunotoxins in MS. The aim of the presented work was the design, production, and characterisation of a novel immunotoxin in the context of MS. Proteins were expressed in a prokaryotic and eukaryotic system, respectively.
Автор: Sigrun K.
Издательство: Книга по Требованию
Год:2010
Цена:
2007 руб
Нет в наличии
Поиск: An Immunotoxin for the Depletion of Autoreactive B Cells in MS. Design, expression, and characterization of an autoimmunotoxin for the specific depletion of autoreactive B cells in multiple sclerosis
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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