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 Branched DNA Assay In the assay, branched DNA is mixed with a sample to be tested. In biology, a branched DNA assay is a test for specific nucleic acid chains, and is
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Branched DNA Assay

автор Frederic P. издательство Книга по Требованию Органическая химия
Cтраниц 104        мягкая обложка       
Аннотация:In the assay, branched DNA is mixed with a sample to be tested. In biology, a branched DNA assay is a test for specific nucleic acid chains, and is typically used to detect retroviruses such as HIV. The assay entirely relies on hybridization. The assay can be run as a "high throughput assay", unlike quantitative Northern-blotting or the RNAse- protection assay, which are labor-intensive and thus difficult to perform on a large number of samples. The detection is done using a non-radioactive method and does not require preamplification of the nucleic acid to be detected. However, the assay can be used to detect and quantify other types of RNA or DNA target. Enzymes are used to indicate the extent of hybridization but are not used to manipulate the nucleic acids. High Quality Content by WIKIPEDIA articles! Thus, small amounts of a nucleic acid can be detected and quantified without a reverse transcription step (in the case of RNA) and/or PCR. The other major high throughput technique employed in the quantification of specific RNA molecules is quantitative PCR, after reverse transcription of the RNA to cDNA.
Автор: Frederic P.
Издательство: Книга по Требованию
Год:2010
Цена:
1521 руб
Нет в наличии
Поиск: Branched DNA Assay
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
КАРНАВАЛ НА НОВЫЙ ГОД РАСПРОДАЖА
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