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 APPLICATION AU CONTROLE DE QUALITE DES GREFFONS MEDULLAIRES CARACTERISATION DES CELLULES MESENCHYMATEUSES NON MANIPULEES.
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APPLICATION AU CONTROLE DE QUALITE DES GREFFONS MEDULLAIRES CARACTERISATION DES CELLULES MESENCHYMATEUSES NON MANIPULEES.

автор Nathalie D. издательство Книга по Требованию Книги
Cтраниц 88        мягкая обложка       
Аннотация:Les cellules mesenchymateuses (CM) ouvrent de seduisantes perspectives pour de nouvelles applications en therapie cellulaire. Quelques differences apparaissent entre CM natives et expandues suggerant une modification in vitro de l' expression de certains antigenes. L' ensemble de ces resultats nous conduit a proposer un phenotypage CD45-CD14-/CD73+ et un test fonctionnel CFU-F pour le Controle de Qualite des cellules mesenchymateuses utilisees en therapie cellulaire, notamment en orthopedie et dans les allogreffes. Leur rarete dans la moelle osseuse implique une selection par adherence et une expansion in vitro systematique. Celle-ci s' accompagne d' une homogeneisation de leurs caracteristiques biologiques laissant supposer que les CM amplifiees ne sont peut-etre pas identiques aux CM natives. Notre travail montre que tous les CFU-F sont inclus dans la population des cellules adherentes CD45-CD14-/GlyA-, ce qui conforte la negativite de l' antigene CD45 sur les CM. En fait, deux sous populations : CD45-CD14- /CD73+ et CD45-CD14-/CD49a+, isolees de moelle fraiche, contiennent l' une et l' autre l' ensemble des progeniteurs mesenchymateux.
Автор: Nathalie D.
Издательство: Книга по Требованию
Год:2010
Цена:
1508 руб
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Поиск: APPLICATION AU CONTROLE DE QUALITE DES GREFFONS MEDULLAIRES CARACTERISATION DES CELLULES MESENCHYMATEUSES NON MANIPULEES.
НОВЫЙ ГОД СИМВОЛ 2013 ГОДАУКРАШЕНИЯ
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